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TECHNICAL ARTICLE
Biodegradation of poly(butylene succinate) by Fusarium sp. FS1301 and purification and characterization of poly(butylene succinate) depolymerase

Publication date: April 2015
Source:Polymer Degradation and Stability, Volume 114

Author(s): Hailong Mao , Huifang Liu , Zhaoying Gao , Tingting Su , Zhanyong Wang

Fusarium sp. FS1301, a poly(butylene succinate) (PBS)-degrading strain, was isolated by screening oil-polluted soil. In this study, the biodegradation behavior of PBS films in the presence of Fusarium sp. FS1301 was investigated. Specifically, the characteristics of PBS films before and after degradation were analyzed. Differential scanning calorimetry and scanning electron microscopy results revealed that both the amorphous and crystalline regions of PBS were degraded by Fusarium sp. FS1301. PBS depolymerase was purified from the culture supernatant of liquid mineral medium with PBS emulsion. The molecular mass of the purified enzyme was determined to be 20kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The optimal pH and temperature for enzyme activity were 8.0 and 50C, respectively. PBS depolymerase maintained its activity from 20C to 50C and from pH 5.0 to 9.0. PBS depolymerase also significantly degraded poly(?-caprolactone) but could not degrade poly(hydroxybutyrate) and poly(lactic acid). Na+ and K+ robustly promoted enzyme activity, whereas EDTA and ?-mercaptoethanol significantly inhibited it. The main degradation products of PBS depolymerase were identified as 1, 4-succinate, succinate-butanediol, succinate-butanediol-succinate, and succinate-butanediol-succinate-butanediol by mass spectrometry. Furthermore, purified PBS depolymerase was found to be identical to cutinase from Fusarium solani by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.





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This project has received funding from the European Unions Seventh Framework Programme for research, technological development and demonstration