'New tailor-made biopolymers produced from lignocellulosic sugars waste for highly demanding fire-resistant applications'

Cost-effective defined medium for the production of polyhydroxyalkanoates using agricultural raw materials

Publication date: October 2015
Source:Bioresource Technology, Volume 194

Author(s): Waranya Suwannasing, Tsuyoshi Imai, Pakawadee Kaewkannetra

According to the cost of carbon substrate for producing biopolymer of polyhydroxyalkanoates (PHAs) still has a barrier to extend in an industrial scale. The aim of this work was to evaluate the defined PHAs media containing the agricultural raw materials of pineapple and sugarcane to produce PHAs by Bacillus strain. Batch fermentation was carried out in flask scale to compare the efficiency of defined media using statistical methodology. The defined medium 8 of pineapple was achieved the highest PHAs concentration and productivity (1.86g/L and 0.077g/Lh). The effect of pH and aeration was extensively studied in a fermentor. The results were revealed that PHAs production would be increased from the condition of uncontrolled pH and at higher aeration rate. The extracted PHAs clearly showed in a homopolymer structure of poly(3-hydroxybutyrate) (PHB) with melting temperature (T m) of 172°C 54.39% crystallinity.

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This project has received funding from the European Unionís Seventh Framework Programme for research, technological development and demonstration